Friday, May 26, 2017

Weekly Reflection for Week of 5/26/17

This week I made 20 plates of agar. Eight plates were made 4pH and the rest (12) were just normal plates which sit at a neutral 7pH. I plan on growing serratia on five of the pH plates, and five of the normal plates. Then I will transfer three of the plain plates to pH plates, and the other two plain plates to another two plain plates, and the pH plates will all be transferred to plain plates. All the plates will then get a disk of the antibiotic tetracycline in the middle. After letting the bacteria grow at room temperature for two days, there was finally enough bacteria to transfer. I was shocked to see that the serratia on the normal plates was pink, and the bacteria on the pH plates was white! I find this very interesting that perhaps pH plays a role on pigment, and after doing research I found that serratia actually grows best at a round 8.5-9 pH. Since I now know this I wonder if antibiotic effectiveness has to do with the level of pigmentation. On Friday I make 30 more plates, this time making three plates at 5, 6, 7, 8, and 9 pH, and the rest regular. I also checked my plates for any rings around the antibiotic, there was nothing today so I will check again Tuesday.

Thursday, May 25, 2017

Weekly Reflection For Week of 5/19/17

This week I focused on research, I’m still very interested in antibiotic resistance and wanted to build off of what I have done the past two years. The past two years I focused on testing pH on E.coli and found that when E.coli was grown on a 4pH agar base the antibiotic (penicillin) was able to kill some of the bacteria. That’s why this year I have decided to see if the same method works on serratia, which is now resistant to tetracycline. Next week I will pour the plates, and test it out.

Friday, April 14, 2017

Weekly Reflection For Week of 4/14/17

This week has been one of my favorite weeks, because it was HEART week!! First off we got to watch a really cool BBC documentary series about surgery, specifically the heart, which was so interesting. I’m looking forward to watching the other episodes. Then of course the next couple of days we got to cut into a deer heart, and understand how all the little valves and arteries come together. I learned so much about the heart this week, and I find it so fascinating how structured the heart is, blood comes in one way and goes out another a specific way. Of course to finish off the week, and make things even better the mouse had her babies!! And a big thanks to Mrs. Cole’s husband for getting all those deer hearts for us!

Weekly Reflection For Week Of 4/7/17

Our week was kicked off by a quiz on inheritance, and then followed by a group project focusing on maintenance physiology, my group was given gas exchange. So for the next couple of days we worked together to figure out why gas exchange is so important and how it works in different organisms. I thought this project was really cool, in the end we had to squeeze all our information onto two pages (we used three), and share it with the class. I thought all the other groups did a really good job, and I learned a lot about different maintenance mechanisms.

Weekly Reflection for Week of 3/31/17

This week we did a really cool lab focusing on different Drosophila (fruit fly) genetics. This was a virtual lab where we were able to pick out the traits of our fruit flies, and mate them. We were then able to see the following generations to figure out if the trait we picked was dominant, recessive, sex-linked or co-dominant. This was a super fun lab, and really helped me better understand all the different kinds of inheritance patterns.

Weekly Reflection for Week of 3/24/17

This week we focused on understanding genetics, and some mutations that can happen by doing a giant cancer activity. In this activity we were all given cards with different kinds of cancer on them, and a description of the kind of gene and its location. We got together in groups and talked about similarities and differences we had. One of the big similarities, which I talk about in my previous post is that the more genes on a chromosome the more common that chromosome will be involved in a mutation. We also did a lot of practice this week with punnett squares, and chi-squares which has really helped me understand the statistical element of genetics. We also did an activity about crossing over, which just showed how much variation can be caused by the event of crossing over.

Monday, March 20, 2017

Cancer Activity 3,2,1

Three Things I Learned From This Activity:
  1. I learned that a certain kind of cancer doesn’t just occur from a mutation in one chromosome, the mutations happen at random spots.
  2. The different mutations don’t effect any one kind of gene, like tumor suppressors, or oncogenes, there’s usually an even mix.
  3. The different mutations don’t effect any one kind gene function, like genome maintenance, genome survival, or genome function. This is random, but it may favor one more than another.
Two Things That Surprised, or Interested Me:
  1. Cancer is a lot more random than I had thought, I figured that one kind of cancer would share a majority of the same mutations, but that wasn't the case. Although there was some shared gene mutations, there weren’t an overwhelming amount to draw conclusions from.  
  2. A lot of the mutations took place on chromosomes, 12, 17, and 14. This makes sense because there are a lot of genes on those chromosomes, but there are also a lot of genes on chromosomes 3 and 4, yet hardly any mutations were found on those chromosomes, so I wonder why that is.
One Question I Still Have:

  1. How many mutations does it take for cancer to appear, does it vary depending on the cancer, or the person?